期刊
ACS NANO
卷 11, 期 11, 页码 11041-11046出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.7b05083
关键词
biosensor; urine exosomes; acute cellular rejection; kidney transplant; proteomics
类别
资金
- NIH [R21-CA205322, R01-HL113156, R01-CA204019, R01-EB010011, R01-EB00462605A1, P01-CA069246]
- Liz Tilberis Award Fund
- MGH scholar fund
- National Research Foundation of Korea [NRF-2017M3A9B4025699, NRF-2017M3A9B4025709]
- Institute for Basic Science [IBS-R026-D1]
- Ministry of Education, South Korea [NRF-2014R1A6A3A03060030]
- American Heart Association [13FTF17000018]
- American Diabetes Association [2016D002888]
- Inflammatory Bowel Disease Grant [DK043351]
- Boston Area Diabetes and Endocrinology Research Center (BADERC) [DK057521]
- National Research Foundation of Korea [2017M3A9B4025709] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Kidney transplant patients require life-long surveillance to detect allograft rejection. Repeated biopsy, albeit the clinical gold standard, is an invasive procedure with the risk of complications and comparatively high cost. Conversely, serum creatinine or urinary proteins are noninvasive alternatives but are late markers with low specificity. We report a urine-based platform to detect kidney transplant rejection. Termed iKEA (integrated kidney exosome analysis), the approach detects extracellular vesicles (EVs) released by immune cells into urine; we reasoned that T cells, attacking kidney allografts, would shed EVs, which in turn can be used as a surrogate marker for inflammation. We optimized iKEA to detect T-cell-derived EVs and implemented a portable sensing system. When applied to clinical urine samples, iKEA revealed high level of CD3-positive EVs in kidney rejection patients and achieved high detection accuracy (91.1%). Fast, noninvasive, and cost-effective, iKEA could offer new opportunities in managing transplant recipients, perhaps even in a home setting.
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