Tunable pH and redox-responsive drug release from curcumin conjugated γ-polyglutamic acid nanoparticles in cancer microenvironment

Tunable pH and redox responsive polymer was prepared using y-polyglutamic acid (gamma-PGA) with linker 3-mercaptopropionic acid (3-MPA) (gamma-PGA_SH) via oxidation to obtain redox responsive disulfide (gamma-PGA_SS) backbone and adipic acid dihydrazide (ADH) (gamma-PGA_SSADH) with hydrazide functional group for pH responsiveness. Further curcumin (Cur) was conjugated through hydrazone bond of the gamma-PGA_SSADH via Schiff base reaction to obtain (gamma-PGA_SSADH_Cur). The prepared systems were characterized by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, Electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Qq-TOF-MS/MS) and Solid state nuclear magnetic resonance (SS NMR) techniques. gamma-PGA_SSADH_Cur formed self-assembled core shell nanoparticles (NPs) in existence of stabilized aqueous medium. gamma-PGA_SSADH_Cur NPs maintained its stability in physiological condition. NPs tunable Cur release and cytotoxicity were observed for gamma-PGA_SSADH_Cur NPs in both acidic and redox conditions mimicking the cancer microenvironment. gamma-PGA_SSADH_Cur NPs uptake study showed via endocytosis mechanism resulted in the lysosomal entrapment of these NPs within the cell. gamma-PGA_SSADH_Cur NPs exhibited a dual stimuli responsive drug delivery and can be used as a smart and potential drug delivery system in cancer microenvironment. (C) 2017 Elsevier B.V. All rights reserved.