期刊
FEMS MICROBIOLOGY LETTERS
卷 364, 期 21, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnx211
关键词
metagenomics; environmental libraries; screening; heterologous expression; industrial enzymes
类别
资金
- European Union [222625]
- MAECI (Ministero Affari Esteri e della Cooperazione Internazionale)
In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely untapped by using traditional isolation and cultivation methods. Metagenomics, which is culture independent, represents a powerful tool for discovering novel enzymes from unculturable microorganisms. Herein, we summarize the variety of approaches adopted for mining environmental DNA and, based on a systematic literature review, we provide a comprehensive list of 332 industrially relevant enzymes discovered from metagenomes within the last three years.
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