期刊
BIORESOURCE TECHNOLOGY
卷 244, 期 -, 页码 534-541出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.07.149
关键词
Polyhydroxyalkanoates; PHB; Halomonas bluephagenesis; gamma-Butyrolactone; Genome engineering
资金
- Ministry of Sciences and Technology [2016YFB0302504]
- National Natural Science Foundation of China [31430003, 31600072]
- Tsinghua President Fund [2015THZ10]
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), short as P(3HB-co-4HB), was successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose and gamma-butyrolactone under open non-sterile conditions. Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding orfZ on plasmids. Fed-batch cultivations conducted in 1-L and 7-L fermentors, respectively, resulted in over 70 g/L cell dry weight (CDW) containing 63% P(3HB-co-12 mol% 4HB) after 48 h under non-sterile conditions. The processes were further scaled up in a 1000-L pilot fermentor to reach 83 g/L CDW containing 61% P(3HB-co-16 mol% 4HB) in 48 h, with a productivity of 1.04 g/L/h, again, under non-sterile conditions. The elastic P(3HB-co-16 mol% 4HB) shows an elongation at break of 1022 +/- 43%. Results demonstrate that the engineered Halomonas bluephagenesis TD01 is a suitable industrial strain for large scale production under open non-sterile conditions.
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