期刊
BIORESOURCE TECHNOLOGY
卷 243, 期 -, 页码 1141-1148出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.07.058
关键词
Lignocellulose; 3-(Hexadecyldimethylammonio) propanesulfonate; Enzymatic hydrolysis; Cellulase; Recover
资金
- National Natural Science Foundation of China [21676109, 21376100]
- Science and Technology Program of Guangzhou [201707020025]
- Guangdong Special Support Plan [2016TX03Z298]
- Guangdong Province Science and Technology Plan [2013B051000011]
Some zwitterionic surfactants exhibit upper critical solution temperature (UCST) in aqueous solutions. For the zwitterionic surfactant solution mixed with cellulase, when its temperature is below UCST, the cellulase can be recovered by coprecipitation with zwitterionic surfactant. In this work, 3-(Hexadecyldimethylammonio) propanesulfonate (SB3-16) was selected to enhance the enzymatic hydrolysis of lignocelluloses and recover the cellulase. After adding 2 mmol/L of SB3-16, the enzymatic digestibility of eucalyptus pretreated by dilute acid (Eu-DA) and by sulfite (Eu-SPORL) increased from 27.9% and 35.1% to 72.6% and 89.7%, respectively. The results showed that SB3-16 could reduce the non-productive adsorption of cellulase on hydrophobic interface, while it did not significantly inhibit the activity of cellulase. For the solution contained 1 wt% SB3-16 and 200 mg protein/L CTec2 cellulase, 55.2% of protein could be recovered by cooling. The filter paper activity of the recovered cellulase was 1.93 FPU/mg protein, which was 95.8% of its initial activity. (C) 2017 Published by Elsevier Ltd.
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