期刊
VIROLOGY JOURNAL
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12985-017-0685-9
关键词
Dengue; Lipid; non-structural protein 3; Fatty acid synthase; Orlistat
类别
资金
- Office of the Higher Education Commission
- Mahidol University under the National Research Universities Initiative
- Mahidol University
- Thailand Research Fund [RTA5780009, IRG5780009]
- Development and Promotion of Science and Technology talents project (DPST)
- Thailand Graduate Institute of Science and Technology (TGIST) Ph.D. Scholarship
- TRF
- Mahidol University (Thai Royal Golden Jubilee) PhD Scholarship
Background: The mosquito transmitted Dengue virus (DENV) remains a significant public health problem in many tropical and subtropical countries. Increasing evidence has suggested that during the infection process cellular lipids play important roles at several stages of the replication cycle. This study sought to characterize the changes in lipid metabolism gene expression and investigated the role of one enzyme, fatty acid synthase, in DENV infection. Methods: Transcriptional profiles of genes associated with lipid metabolism were evaluated by real-time PCR after infection of different cell lines (HepG2 and HEK293T/17) and with different DENVs (laboratory adapted and low passage). Expression profiles of genes were evaluated by western blotting. A critical lipid metabolism protein, fatty acid synthase was down-regulated through siRNA and inhibited with orlistat and the effect on DENV infection determined by flow cytometry, plaque assay, western blotting and confocal microscopy. Results: The results showed alterations of gene transcription and expression were seen in genes variously associated with lipogenesis, lipolysis and fatty acid beta-oxidation during DENV infection. Interference of fatty acid synthase with either siRNA or orlistat had marked effects on virus production, with orlistat having an EC50 value of 10.07 mu M at 24 h post infection. However, non-structural protein expression was largely unaffected. Conclusions: While drug treatment reduced virus titer by up to 3Log10, no significant effect on DENV non-structural protein expression was observed, suggesting that fatty acid synthase acts through an effect on virion formation.
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