期刊
VIROLOGY
卷 500, 期 -, 页码 103-113出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.10.013
关键词
Chloroviruses; Algal viruses; Chlorella variabilis; OSy-NE5; Permissive cells; Non-permissive cells
类别
资金
- NSF-EPSCoR Grant [EPS-1004094]
- COBRE program of the National Center for Research Resources Grant [P20-RR15535]
- National Center for Research Resources grant [5P20RR016469]
- National Institute for General Medical Science grant [8P20GM103427]
- UNL UCARE scholarship
- ARD scholarship
- Egyptian Ministry of Higher Education (Cultural Affairs and Missions Sector)
- USDA/ARS [5438-31320-012-00D]
A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2-3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2-3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2-3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical 2612 base-paired inverted sequence after which the DNA sequence diverged. OSy-NE5 encoded 357 predicted CDSs and 13 tRNAs. Interestingly, OSy-NE5 attached to and initiated infection in NC64A cells but infectious progeny viruses were not produced; thus OSy-NE5 replication in NC64A is blocked at some later stage of replication.
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