期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 46, 页码 14556-14560出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201708277
关键词
bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring
资金
- Ecole Polytechnique Federale de Lausanne
- Swiss National Science Foundation
- National Centre of Competence in Research Chemical Biology
- Max Planck Society
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.
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