期刊
VETERINARY PARASITOLOGY
卷 247, 期 -, 页码 19-25出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2017.09.015
关键词
Toxoplasma gondii; Genotypes; ROP18; ROP5; Virulence; Pig; Goat
资金
- Conselho Nacional de Desenvolvimento e Pesquisa (CNPq)
- Fundacao de Amparo a Pesquisa de Minas Gerais (FAPEMIG)
- Pro-Reitoria de Pesquisa of Universidade Federal de Minas Gerais
- CAPES
- CNPq [152652/2016-7, 303770/2013-9]
Recent studies indicate that proteins GRA15, ROP5, ROP16, ROP17, and ROP18 of Toxoplasma gondii are involved in the process of interaction, cellular invasion, and immune response of the host. Among these proteins, alleles of the polymorphic ROP18 and ROP5 seem to be directly associated with T. gondii virulence in mice. The purpose of this work was to isolate and genotype T. gondii from pig, goat, and sheep slaughtered for human consumption in the state of Piaui, Northeastern Brazil and relate the variability of genes that express virulence proteins of the parasite to virulence in mice. T. gondii was isolated from 16 pigs and 9 goats. The parasite was not isolated from sheep samples. Eleven different genotypes were identified using PCR-RFLP. A unique genotype not yet described in any other host and or anywhere else was common to three pig isolates. Eighteen isolates (72%) were characterized as avirulent, four (16%) as intermediate virulence and three (12%) as virulent to mice. The combined analysis of ROP18 and ROP5 in the isolates studied in Piaui, showed four different allele associations: 4/3 (virulent strains), 3/3, 3/1, and 2/3 (avirulent strains). The association 2/3 was not previously described in the literature. Our results indicated that GRA15, ROP16, and ROP17 alleles were not associated with T. gondii virulence in mice. Pigs and goats raised and slaughtered for human consumption in the state of Piaui are infected with isolates of T. gondii presenting different genotypes. We concluded that the virulence protein ROP18, analyzed alone or in combination with ROP5, was effective in determining virulence for mice for the new isolates of T. gondii.
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