期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 77, 期 -, 页码 88-94出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2017.07.025
关键词
Infectious hematopoietic necrosis virus; Autophagy; LC3; Antiviral response
资金
- Central-Level Non-profit Scientific Research Institutes Special Funds [HSY201410, 2016HY-ZD0504]
- Heilongjiang Province Research and Development of Applied Technology [GA13B401]
Infectious hematopoietic necrosis virus (IHNV) is a common pathogen that causes severe disease in the salmonid aquaculture industry. Recent work demonstrated that autophagy plays an important role in pathogen invasion by activating innate and adaptive immunity. This study investigated the relationship between IHNV and autophagy in epithelioma papulosum cyprini cells. The electron microscopy results show that IHNV infection can induce typical autophagosomes which are representative structures of autophagy activation. The punctate accumulation of green fluorescence-tagged microtubule-associate protein 1 light chain 3 (LC3) and the protein conversion from LC3-I to LC3-II were respectively confirmed by confocal fluorescence microscopy and western blotting. Furthermore, the effects of autophagy on IHNV replication were also clarified by altering the autophagy pathway. The results showed that rapamycin induced autophagy can inhibit both intracellular viral replication and extracellular viral yields, while autophagy inhibitor produced the opposite results. These findings demonstrated that autophagy plays an antiviral role during IHNV infection. (C) 2017 Elsevier Ltd. All rights reserved.
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