期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 409, 期 28, 页码 6567-6574出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0605-2
关键词
Smartphone; Zearalenone; Enzyme-linked immunosorbent assays; Colorimetric reader; Ambient light sensor
资金
- National Key Development Program of China [2016YFD0500600]
- National Key Technology R D Program [2008BAK42B-05]
- Guangdong Province Key Scientific Research [2013A022100031]
Smartphone biosensors could be cost-effective, portable instruments to be used for the readout of liquid colorimetric assays. However, current reported smartphone colorimetric readers have relied on photos of liquid assays captured using a camera, and then analyzed using software programs. This approach results in a relatively low accuracy and low generality. In this work, we reported a novel smartphone colorimetric reader that has been integrated with an ambient light sensor and a 3D printed attachment for the readout of liquid colorimetric assays. The portable and low-cost ($0.15) reader utilized a simplified electronic and light path design. Furthermore, our reported smartphone colorimetric reader can be compatible with different smartphones. As a proof of principle, the utility of this device was demonstrated using it in conjunction with an enzyme-linked immunosorbent assay to detect zearalenone. Results were consistent with those obtained using a professional microplate reader. The developed smartphone colorimetric reader was capable of providing scalable, cost-effective, and accurate results for liquid colorimetric assays that related to clinical diagnoses, environment pollution, and food testing.
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