期刊
BRAIN BEHAVIOR AND IMMUNITY
卷 66, 期 -, 页码 125-134出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2017.07.011
关键词
Synapsin; Antibodies; Psychiatric disorders; Neurologic disorders; Demyelinating disease; Autoimmunity; Serum
资金
- Deutsche Forschungsgemeinschaft (DFG)
- German Ministry of Education and Research (BMBF/KKNMS, Competence Network Multiple Sclerosis)
- Deutsche Forschungsgemeinschaft [DFG EXC 257]
- Israel Science Foundation (ISF) [1427/12]
Objective: To study the prevalence of autoantibodies to synapsin in patients with psychiatric and neurological disorders and to describe clinical findings in synapsin antibody positive patients. Methods: Sera of 375 patients with different psychiatric and neurological disorders and sera of 97 healthy controls were screened (dilution 1:320) for anti-synapsin IgG using HEK293 cells transfected with rat synapsin Ia. Positive sera were further analyzed by immunoblots with brain tissue from wild type and synapsin knock out mice and with HEK293 cells transfected with human synapsin Ia and lb. Binding of synapsin IgG positive sera to primary neurons was studied using murine hippocampal neurons. Results: IgG in serum from 23 (6.1%) of 375 patients, but from none of the 97 healthy controls (p = 0.007), bound to rat synapsin Ia transfected cells with a median (range) titer of 1:1000 (1:320-1:100,000). Twelve of the 23 positive sera reacted with a protein of the molecular size of synapsin I in immunoblots of wild type but not of synapsin knock out mouse brain tissue. Out of 19/23 positive sera available for testing, 13 bound to human synapsin Ia and 16 to human synapsin lb transfected cells. Synapsin IgG positive sera stained fixed and permeabilized murine hippocampal neurons. Synapsin IgG positive patients had various psychiatric and neurological disorders. Tumors were documented in 2 patients (melanoma, small cell lung carcinoma); concomitant anti-neuronal or other autoantibodies were present in 8 patients. Conclusions: Autoantibodies to human synapsin la and Ib are detectable in a proportion of sera from patients with different psychiatric and neurological disorders, warranting further investigation into the potential pathophysiological relevance of these antibodies. (C) 2017 Elsevier Inc. All rights reserved.
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