Molecular basis for increased susceptibility of Indigenous North Americans to seropositive rheumatoid arthritis

Objective The pathogenetic mechanisms by which HLA-DRB1 alleles are associated with anticitrullinated peptide antibody (ACPA)-positive rheumatoid arthritis (RA) are incompletely understood. RA high-risk HLA-DRB1 alleles are known to share a common motif, the 'shared susceptibility epitope (SE)'. Here, the electropositive P4 pocket of HLA-DRB1 accommodates self-peptide residues containing citrulline but not arginine. HLA-DRB1 His/Phe13 beta stratifies with ACPA-positive RA, while His13 beta Ser polymorphisms stratify with ACPA-negative RA and RA protection. Indigenous North American (INA) populations have high risk of early-onset ACPA-positive RA, whereby HLA-DRB1* 04: 04 and HLA-DRB1* 14: 02 are implicated as risk factors for RA in INA. However, HLA-DRB1* 14: 02 has a His13 beta Ser polymorphism. Therefore, we aimed to verify this association and determine its molecular mechanism. Methods HLA genotype was compared in 344 INA patients with RA and 352 controls. Structures of HLA-DRB1* 1402-class II loaded with vimentin-64Arg(59-71), vimentin-64Cit(59-71) and fibrinogen beta-74Cit(69-81) were solved using X-ray crystallography. Vimentin-64Cit(59-71) -specific and vimentin(59-71)-specific CD4(+) T cells were characterised by flow cytometry using peptide-histocompatibility leukocyte antigen (pHLA) tetramers. After sorting of antigen-specific T cells, TCRa and beta-chains were analysed using multiplex, nested PCR and sequencing. Results ACPA+ RA in INA was independently associated with HLA-DRB1*14: 02. Consequent to the His13 beta Ser polymorphism and altered P4 pocket of HLA-DRB1* 14: 02, both citrulline and arginine were accommodated in opposite orientations. Oligoclonal autoreactive CD4+ effector T cells reactive with both citrulline and arginine forms of vimentin 59-71 were observed in patients with HLA-DRB1* 14: 02+ RA and at-risk ACPA-first-degree relatives. HLA-DRB1* 14: 02vimentin(59-71)-specific and HLA-DRB1*14: 02-vimentin64Cit 59-71 -specific CD4+ memory T cells were phenotypically distinct populations. Conclusion HLA-DRB1* 14: 02 broadens the capacity for citrullinated and native self-peptide presentation and T cell expansion, increasing risk of ACPA+ RA.