期刊
BRAIN BEHAVIOR AND IMMUNITY
卷 66, 期 -, 页码 193-200出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2017.06.011
关键词
Flow cytometry; Depression; Sleep; Regulatory T cells; CD8(+) cells; Natural killer cells
资金
- National Institute of Mental Health, National Institutes of Health, United States [1R01MH0908099]
A subset of individuals with major depressive disorder (MDD) have impaired adaptive immunity characterized by a greater vulnerability to viral infection and a deficient response to vaccination along with a decrease in the number and/or activity of T cells and natural killer cells (NKC). Nevertheless, it remains unclear which specific subsets of lymphocytes are altered in MDD, a shortcoming we address here by utilizing an advanced fluorescence-activated cell sorting (FACS) method that allows for the differentiation of important functionally-distinct lymphocyte sub-populations. Furthermore, despite evidence that sleep disturbance, which is a core symptom of MDD, is itself associated with alterations in lymphocyte distributions, there is a paucity of studies examining the contribution of sleep disturbance on lymphocyte populations in MDD populations. Here, we measured differences in the percentages of 13 different lymphocytes and 6 different leukocytes in 54 unmedicated MDD patients (partially remitted to moderate) and 56 age and sex-matched healthy controls (HC). The relationship between self-reported sleep disturbance and cell counts was evaluated in the MDD group using the Pittsburgh Sleep Quality Index(PSQI). The MDD group showed a significantly increased percentage of CD127(low)/CCR4+ Treg cells, and memory Treg cells, as well as a reduction in CD56(+)CD16(-) (putative immunoregulatory) NKC counts, the latter, prior to correction for body mass index. There also was a trend for higher effector memory CDS+ cell counts in the MDD group versus the HC group. Further, within the MDD group, self-reported sleep disturbance was associated with an increased percentage of effector memory CDS+ cells but with a lower percentage of CD56+CD16- NKC. These results provide important new insights into the immune pathways involved in MDD, and provide novel evidence that MDD and associated sleep disturbance increase effector memory CD8(+) and Treg pathways. Targeting sleep disturbance may have implications as a therapeutic strategy to normalize NKC and memory CDS' cells in MDD. (C) 2017 Elsevier Inc. All rights reserved.
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