期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 183, 期 3, 页码 807-819出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-017-2465-9
关键词
Candida antarctic lipase B; Ionic liquids; Carbon nanotubes; Modification; Enzyme immobilization
资金
- National Natural Science Foundation of China [21676143]
- Qing Lan Project
- National Science Fund for Distinguished Young Scholars [21225626]
Multiwalled carbon nanotubes (MWNTs) were modified by imidazole-based ionic liquids with different alkyl groups. The modified support samples were characterized by scanning transmission electron microscopy, Raman spectra, thermogravimetric analyses, and X-ray photoelectron spectroscopy. The samples were used to immobilize Candida antarctic lipase (CALB) and the influence of alkyl chain length of ionic liquids on enzymatic properties was investigated by the hydrolysis reaction of triacetin. The results revealed that functionalized ionic liquids modification did not destroy the structure of MWNTs. Compared with the immobilized CALB on MWNTs, the immobilized CALB on novel carriers all exhibited higher activity, thermal stability, and reusability. Especially, the activity of MWNTs-IL (8C)-CALB improved 15.23-folds than MWNTs-CALB, meanwhile, after incubation at 70 A degrees C for 20 min, residual enzyme activity of MWNTs-IL (8C)-CALB was 46% of the initial activity, while MWNTs-CALB already lost all activity. Besides, MWNTs-IL (8C)-CALB retained 64.5% of its initial activity after 4 cycles, while MWNTs-CALB retained only 2.12%.
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