期刊
BIORESOURCE TECHNOLOGY
卷 245, 期 -, 页码 1447-1454出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.05.088
关键词
Saccharomyces cerevisiae; Consolidated bioprocessing (CBP); Artificial zinc-finger protein (AZFP); Thermotolerance; Jerusalem artichoke stalk (JAS); Cellulosic bioethanol production
资金
- National Science Foundation of China [31461143029, 21376043, 5151101168]
- Chinese Scholarship Council (CSC)
In this work, the consolidated bioprocessing (CBP) yeast Saccharomyces cerevisiae MNII/cocdBEC3 was transformed by an artificial zinc finger protein (AZFP) library to improve its thermal tolerance, and the strain MNII-AZFP with superior growth at 42 degrees C was selected. Improved degradation of acid swollen cellulose by 45.9% led to an increase in ethanol production, when compared to the control strain. Moreover, the fermentation of Jerusalem artichoke stalk (JAS) by MNII-AZFP was shortened by 12 h at 42 degrees C with a concomitant improvement in ethanol production. Comparative transcriptomics analysis suggested that the AZFP in the mutant exerted beneficial effect by modulating the expression of multiple functional genes. These results provide a feasible strategy for efficient ethanol production from JAS and other cellulosic biomass through CBP based-fermentation at elevated temperatures. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据