期刊
CHEMBIOCHEM
卷 18, 期 19, 页码 1898-1902出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201700410
关键词
electrochemistry; exocytosis; membranes; solvent effects; vesicles
资金
- European Research Council
- Knut and Alice Wallenberg Foundation
- Swedish Research Council (VR)
- National Institutes of Health
Dimethyl sulfoxide (DMSO) is frequently used as a solvent in biological studies and as a vehicle for drug therapy; but the side effects of DMSO, especially on the cell environment, are not well understood, and controls with DMSO are not neutral at higher concentrations. Herein, electrochemical measurement techniques are applied to show that DMSO increases exocytotic neurotransmitter release, while leaving vesicular contents unchanged. In addition, the kinetics of release from DMSO-treated cells are faster than that of untreated ones. The results suggest that DMSO has a significant influence on the chemistry of the cell membrane, leading to alteration of exocytosis. A speculative chemical mechanism of the effect on the fusion pore during exocytosis is presented.
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