期刊
CHEMBIOCHEM
卷 18, 期 19, 页码 1893-1897出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201700331
关键词
biosynthesis; carmine; enzymes; polyketides; synthetic biology
资金
- Innovation Fund Denmark [018-2011-1]
- Lundbeck Foundation [R223-2016-85] Funding Source: researchfish
- Villum Fonden [00007523] Funding Source: researchfish
Carminic acid is a C-glucosylated octaketide anthraquinone and the main constituent of the natural dye carmine (E120), possessing unique coloring, stability, and solubility properties. Despite being used since ancient times, longstanding efforts to elucidate its route of biosynthesis have been unsuccessful. Herein, a novel combination of enzymes derived from a plant (Aloe arborescens, Aa), a bacterium (Streptomyces sp. R1128, St), and an insect (Dactylopius coccus, Dc) that allows for the biosynthesis of the C-glucosylated anthraquinone, dcII, a precursor for carminic acid, is reported. The pathway, which consists of AaOKS, StZhuI, StZhuJ, and DcUGT2, presents an alternative biosynthetic approach for the production of polyketides by using a typeIII polyketide synthase (PKS) and tailoring enzymes originating from a typeII PKS system. The current study showcases the power of using transient expression in Nicotiana benthamiana for efficient and rapid identification of functional biosynthetic pathways, including both soluble and membrane-bound enzymes.
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