期刊
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
卷 240, 期 6, 页码 1129-1133出版社
SPRINGER
DOI: 10.1007/s00217-015-2416-z
关键词
Donax species; Species identification; PCR-RFLP; COI
资金
- Conselleria de Economia e Industria (Xunta de Galicia) [10MMA103013]
- Ministerio de Educacion of Spain
Four Donax species, D. semistriatus, D. trunculus, D. variegatus and D. vittatus, are found on European coasts. Nevertheless, despite their economic importance there is not a reliable method to differentiate these species independently of their size or condition. Such a method could help to protect consumer rights and avoid commercial fraud due to the replacement of valuable species by less valuable ones with similar morphological traits. In this work, the sequence of the mitochondrial cytochrome oxidase subunit I region was examined in individuals of these species to identify restriction site variation and develop polymerase chain reaction-restriction fragment length polymorphisms assays. Species-specific restriction endonuclease patterns were found with the enzymes AluI, HaeIII and MspI, allowing an exact identification of Donax species. This methodology provides simple, reliable and cost-effective identification of four Donax species and may be useful to prevent commercial fraud and to increase food traceability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据