期刊
CHINESE JOURNAL OF CANCER RESEARCH
卷 29, 期 5, 页码 463-470出版社
CHINESE JOURNAL CANCER RESEARCH CO
DOI: 10.21147/j.issn.1000-9604.2017.05.11
关键词
Acute myeloid leukemia; PD-1; T cell exhaustion
类别
资金
- National Natural Science Foundation of China [81570143, 91642111]
- Guangdong Provincial Basic Research Program [2015B020227003]
- Guangdong Provincial Applied Science and Technology Research & Development Program [2016B020237006]
- Guangzhou Science and Technology Project [201510010211]
- Fundamental Research Funds for the Central Universities [21616108]
- Medical Scientific Research Foundation of Guangdong Province, China [A2016045]
Objective: To investigate the association between the T cell inhibitory receptor programmed death 1 (PD-1) and T cell exhaustion status in T cells from patients with de novo acute myeloid leukemia (AML) and AML in complete remission (CR). Methods: Surface expression of PD-1 and the exhaustion and immunosenescence markers CD244 and CD57 on CD3+, CD4+ and CD8+ T cells from peripheral blood samples from 20 newly diagnosed, untreated AML patients and 10 cases with AML in CR was analyzed by flow cytometry. Twenty-three healthy individuals served as control. Results: A significantly higher percentage of PD-1+ cells were found for CD3+ T cells in the de novo AML group compared with healthy controls. In addition, an increased level of PD-1+ CD8+ T cells, but not PD-1+ CD4+, was found for CD3+ T cells in the de novo AML and AML-CR samples. A higher percentage of CD244+CD4+, CD244+CD8+, CD57+CD4+ and CD57+CD8+ T cells was found in CD3+ T cells in samples from those with de novo AML compared with those from healthy controls. Strong increased PD-1+CD244+ and PD-1+CD57+ co-expression was found for CD4+ and CD8+ T cells in the de novo AML group compared with healthy controls. Conclusions: We characterized the major T cell defects, including co-expression of PD-1 and CD244, CD57-exhausted T cells in patients with de novo AML, and found a particular influence on CD8+ T cells, suggesting a poor anti-leukemia immune response in these patients.
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