期刊
ANALYTICAL CHEMISTRY
卷 89, 期 22, 页码 12569-12577出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b03884
关键词
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资金
- China Postdoctoral Science Foundation
- National Natural Science Foundation of China [31600076, 31470220, 31670101, 31327001, 31601084, 21775155, 31425002]
- Ministry of Science and Technology of China [2016YFF0202303]
- Chinese Academy of Sciences [KFJ-SW-STS-165, XDBP0305]
- China Postdoctoral Science Foundation [2017M612364, 2017T100522]
- Shandong Province Natural Science Foundation [ZR2014CQ005]
- State Development & Investment Corporation (SDIC)
Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput remains one major factor limiting its broader application. Here we present an integrated Raman-activated droplet sorting (RADS) microfluidic system for functional screening of live cells in a label-free and high-throughput manner, by employing AXT-synthetic industrial microalga Haematococcus pluvialis (H. pluvialis) as a model. Raman microspectroscopy analysis of individual cells is carried out prior to their microdroplet encapsulation, which is then directly coupled to DEP-based droplet sorting. To validate the system, H. pluvialis cells containing different levels of AXT were mixed and underwent RADS. Those AXT-hyperproducing cells were sorted with an accuracy of 98.3%, an enrichment ratio of eight folds, and a throughput of similar to 260 cells/min. Of the RADS-sorted cells, 92.7% remained alive and able to proliferate, which is equivalent to the unsorted cells. Thus, the RADS achieves a much higher throughput than existing RACS systems, preserves the vitality of cells, and facilitates seamless coupling with downstream manipulations such as single-cell sequencing and cultivation.
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