期刊
ULTRASONICS SONOCHEMISTRY
卷 37, 期 -, 页码 1-8出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2016.12.024
关键词
Low-frequency and low-intensity; ultrasound; M. smegmatis; Permeability; Bactericidal effect; Levofloxacin
资金
- Chongqing Research Program of Basic Research and Frontier Technology [csct2016jcyjA0098]
- Chongqing Science & Technology Commission [cstc2016shm-szx130029]
- National Natural Science Fund
- Chinese National Science Foundation [81127901, 31571453, 11574039]
Tuberculosis is an infectious disease caused by the bacterium M. tuberculosis. The aim of this study was to investigate the bactericidal effect and underlying mechanisms of low-frequency and low-intensity ultrasound combined with levofloxacin treatment against M. smegmatis (a surrogate of M. tuberculosis). As part of this study, M. smegmatis was continuously irradiated with low frequency ultrasound (42 kHz) using several different doses whereby both intensity (0.138, 0.190 and 0.329 W/cm(2)) and exposure time (5, 15 and 20 min) were varied. Flow cytometric analyses revealed that the permeability of M. smegmatis increased following ultrasound exposure. The survival rate, structure and morphology of bacteria in the lower-dose (I-SATA = 0.138 W/cm(2) for 5 min) ultrasound group displayed no significant differences upon comparison with the untreated group. However, the survival rate of bacteria was significantly reduced and the bacterial structure was damaged in the higher-dose (I-SATA = 0.329 W/cm(2) for 20 min) ultrasound group. Ultrasound irradiation (0.138 W/cm(2)) was subsequently applied to M. smegmatis in combination with levofloxacin treatment for 5 min. The results demonstrated that the bactericidal effect of ultrasonic irradiation combined with levofloxacin is higher compared to ultrasound alone or levofloxacin alone. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据