期刊
CARBOHYDRATE RESEARCH
卷 452, 期 -, 页码 122-128出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2017.10.001
关键词
Sialylglycopeptide; N-glycan; Isolation; Egg yolk powder; HILIC-HPLC
资金
- National Institute of General Medical Sciences [U01GM120408, R01GM090269, P01GM107012]
- National Heart, Lung, and Blood Institute [P01HL107150]
- National Cancer Institute from the US National Institutes of Health (NIH) [F31CA180478]
- NIH [S10RR027097]
Sialylglycopeptide (SGP) is a complex bi-antennary N-glycan bearing a short peptide fragment that can be isolated from the yolk of hen eggs. This natural product has gained popularity as a starting material for the semi-synthesis of N-glycans. We have found that current isolation methods provide a glycopeptide contaminated with several related structures, one being a glycopeptide having a hexose directly attached to peptide backbone, most like through the hydroxyl containing side chain of the threonine moiety. Furthermore, current methods employ fresh egg yolks that need to be lyophilized and involve several tedious purification steps. Herein, we report a convenient method for the isolation of gram quantities of homogeneous SGP from commercially available egg yolk powder using solid/ liquid extraction and HILICHPLC purification. (C) 2017 Elsevier Ltd. All rights reserved.
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