期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 43, 期 1, 页码 383-394出版社
KARGER
DOI: 10.1159/000480417
关键词
Mir-210; H2O2; ISCU; Oxidative stress; Energy metabolism shift
资金
- National Natural Science Foundation of China [81570250]
- Bethune Medical Department [yb201503]
- Scientific and Technological Department of Jilin Province [20150204008YY]
- National Clinical Key Specialty Project
Background/Aims: The myocardial energy metabolism shift is one of the most important pathological features of ischemic heart disease (IHD). Although several microRNAs (miRs) are involved in the regulation of myocardial energy metabolism, their exact effects and underlying mechanisms remain unclear. The aim of this study was to investigate whether microRNA(miR-210) regulates the energy metabolism shift during oxidative stress in H9c2 cardiomyocytes. Methods: Cell survival was analyzed via CCK assay. The energy metabolism shift was detected by lactate assay, ATP assay and RT2 profiler glucose metabolism PCR array. Protein and mRNA expression levels were determined by western blot and qPCR. We also used kits to detect the activity of Complex I, Sirt3 and the NAD+/NADH ratio. Results: We determined that miR-210 promoted the energy metabolism shift. The iron-sulfur cluster assembly protein (ISCU) was a target of miR-210. Additionally, we detected the activity of complex I and found that miR-210 inhibits mitochondrial respiration. Interestingly, miR-210 may also indirectly regulate SIRT3 by regulating ISCU. Conclusion: Our results confirm that miR-210 is essential and sufficient for modulating the cellular energy metabolism shift during H2O2-induced oxidative stress in H9c2 cardiomyocytes by targeting ISCU. (C) 2017 The Author(s) Published by S. Karger AG, Basel
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