期刊
ANALYTICAL CHEMISTRY
卷 89, 期 21, 页码 11685-11689出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b03133
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资金
- National Key R&D Program of China [2017YFC0906500]
- National Natural Science Foundation of China [21421004, 21327807, 21707035]
- Fundamental Research Funds for the Central Universities [222201718001, 222201717003]
Detection of DNA methylation In real human serum is of great importance to push the development of clinical research and early diagnosis of human diseases: Herein, taking advantage of stable pore structure of aerolysin hi a harsh environment, we distinguish methylated cytosine from cytosine using aerolysin nanopore in human serum. Since wild-type (WT) aerolysin enables high sensitivity detection of DNA, the subtle difference between methylated cytosine and cytosine could be measured directly without any specific designs. Methylated cytosine induced, a population of I/I-0 = 0.53 while cytosine was focused on I/I-0 = 0.56. The dwell time of methylated cytosine (5.3 +/- 0.1 ms) was much longer than that of cytosine (3.9 +/- 0.1 ms), which improves the accuracy for the discrimination of the two oligomers. Moreover, the pore-membrane system could remain stable for more than 2 h and achieve the detection of Methylated cytosine with zero-background signal in the presence of serum. Additionally, event frequency of methylated cytosine is in Correspondence with the relative concentration and facilitate the quantification of methylation.
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