期刊
CYTOLOGIA
卷 82, 期 5, 页码 559-562出版社
UNIV TOKYO CYTOLOGIA
DOI: 10.1508/cytologia.82.559
关键词
CRISPR-dCas9; Chromatin dynamics; Live cell imaging; Nicotiana tabacum
资金
- JST CREST [JPMJCR-13B4]
- MEXT/JSPS KAKENHI [JP15H5955, JP15K21750, JP15H05962]
- Grants-in-Aid for Scientific Research [15H05962] Funding Source: KAKEN
Labeling of specific DNA sequences in living organisms is important for understanding the mechanism of gene regulation during development, differentiation, and environmental adaptation. Here we describe a CRISPR/Cas9-based chromatin visualization system in plants. We adapted the mammalian nuclease-dead Cas9-fluorescent protein (dCas9-FP) system to a plant expression vector. Transient expression of dCas9-FP and single guide RNA (sgRNA) for telomeric sequences efficiently labeled telomere repeats in tobacco.
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