期刊
CHEMOSPHERE
卷 188, 期 -, 页码 337-344出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2017.09.008
关键词
Microalgal bioassay; Chlamydomonas reinhardtii; Alkaline phosphatase; Hydrodynamic voltammetry; Droplet; Rotating disk electrode
资金
- Grants-in-Aid for Scientific Research [16K05820, 16H02976] Funding Source: KAKEN
We developed an electrochemical microalgal bioassay for the determination of heavy metal toxicity in water on the basis of the alkaline phosphatase (ALP) enzyme inhibition of Chlamydomonas reinhardtii. Five heavy metals were chosen as toxicants: Hg, Cd, Pb, Zn, and Cu. The induced ALP activity of C reinhardtii was inhibited using the phosphate starvation method, and the results were evaluated by measuring the electrochemical oxidation of p-aminophenol (PAP) following the enzymatic conversion of p-aminophenyl phosphate (PAPP) as a substrate. The rapid determination,of enzymatic activity was achieved using hydrodynamic voltammetry in a 50 mu L. micro-droplet with a rotating disk electrode (RDE). Enzymatic activity over a PAPP substrate is affected by heavy metal ions, and this phenomenon decreases the chronoamperometric current signal. The concentrations of Hg, Cd, Pb, Zn, and Cu in which the ALP activity was half that of the control (EC50) were found to be 0.017, 0.021, 0.27, 1.30, and 136 respectively. The RDE system was demonstrated to be capable of detecting enzymatic activity by using a small amount of regent, a reaction time of only 60 s, and a detection limit of 5.4 x 10(-7) U. (C) 2017 Elsevier Ltd. All rights reserved.
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