Morphofunctional Properties of Human Platelets Treated with Silver Nanoparticles

We studied structural and functional properties of human platelets in the presence of nanosilver particles. Incubation with 0.05-5 mu M silver nanoparticles suppressed platelet adhesion in a dose-dependent manner without affecting internal platelet structure; during adhesion, some granules were not exocytized. Spontaneous platelet activation was observed at nanoparticle concentrations 15-100 mu M. Addition of 1-5 mu M nanosilver to cells undergoing adhesion blocked massive platelet degranulation, but did not prevent the formation of lamellopodia. The maximum number of preserved granules in platelets was revealed in the presence of 2.5-5 mu M silver nanoparticles: 50% after platelet preincubation with silver nanoparticles and 75-77% after stabilization of adherent platelets with silver nanoparticles.