期刊
BIORESOURCE TECHNOLOGY
卷 245, 期 -, 页码 1304-1313出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.05.031
关键词
Biocatalysts; Directed evolution; Immobilisation; Nanoparticles; Site directed mutagenesis
资金
- Department of Biotechnology under DBT Research Associateship programme
- Department of Biotechnology under DBT Bio-CARe scheme
Biocatalysts are creating increased interest among researchers due to their unique properties. Several enzymes are efficiently produced by microorganisms. However, the use of natural enzymes as biocatalysts is hindered by low catalytic efficiency and stability during various industrial processes. Many advanced enzyme technologies have been developed to reshape the existing natural enzymes to reduce these limitations and prospecting of novel enzymes. Frequently used enzyme technologies include protein engineering by directed evolution, immobilisation techniques, metagenomics etc. This review summarizes recent and emerging advancements in the area of enzyme technologies for the development of novel biocatalysts and further discusses the future directions in this field. (C) 2017 Elsevier Ltd. All rights reserved.
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