期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 180, 期 2, 页码 327-337出版社
HUMANA PRESS INC
DOI: 10.1007/s12011-017-1001-7
关键词
AgNPs; Bombyx mori; Environmental risk; Fat body proteins; Growth
资金
- National Natural Science Foundation of China [31572467]
- Research Fund for International Young Scientists of China [31550110210]
Ag nanoparticles (AgNPs), a widely used non-antibiotic, antibacterial material, have shown toxic and other potentially harmful effects in mammals. However, the deleterious effects of AgNPs on insects are still unknown. Here, we studied the effects of AgNPs on the model invertebrate organism Bombyx mori. After feeding silkworm larvae different concentrations of AgNPs, we evaluated the changes of B. mori body weights, survival rates, and proteomic differences. The results showed that low concentrations (< 400 mg/L) of AgNPs promoted the growth and cocoon weights of B. mori. Although high concentrations (>= 800 mg/L) of AgNPs also improved B. mori growth, they resulted in silkworm death. An analysis of fat body proteomic differences revealed 13 significant differences in fat body protein spots, nine of which exhibited significantly downregulated expression, while four showed significantly upregulated expression. Reverse transcription-polymerase chain reaction results showed that at an AgNP concentration of 1600 mg/L, the expression levels of seven proteins were similar to the transcription levels of their corresponding genes. Our results suggest that AgNPs lowered the resistance to oxidative stress, affected cell apoptosis, and induced cell necrosis by regulating related protein metabolism and metabolic pathways in B. mori.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据