期刊
BIORESOURCE TECHNOLOGY
卷 245, 期 -, 页码 560-566出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.08.132
关键词
Assimilation of acetate; Product shift; Carbon monoxide fermentation; Eubacterium limosum KIST612
资金
- Center for C1 Gas Refinery Program through the National Research Foundation of Republic of Korea [NRF-2015M3D3A1A01064883]
- National Research Foundation of Korea [2015M3D3A1A01064883] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The acetate-assisted cultivation of Eubacterium limosum KIST612 was found to provide a way for enhancing cell mass, the carbon monoxide (CO) consumption rate, and butyrate production using CO as an electron and energy source. Cell growth (146%), mu(max) (121%), and CO consumption rates (151%) increased significantly upon the addition of 30 mM acetate to microbial cultures. The main product of CO fermentation by E. limosum KIST612 shifted from acetate to butyrate in the presence of acetate, and 5.72 mM butyrate was produced at the end of the reaction. The resting cell experimental conditions indicated acetate uptake and an increase in the butyrate concentration. Three routes to acetate assimilation and energy conservation were suggested based on given experimental results and previously genome sequencing data. Acetate assimilation via propionate CoA-transferase (PCT) was expected to produce 1.5 mol ATP/mol butyrate, and was thus anticipated to be the most preferred route.
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