期刊
BIORESOURCE TECHNOLOGY
卷 245, 期 -, 页码 274-282出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.08.004
关键词
Wax esters; Microbial oil; Food processing waste and by-products; Lipase; Oleochemicals
资金
- National Agency of Petroleum (ANP)
- Petrobras (Brazil)
- Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ)
- National Council for Scientific and Technological Development of the Ministry of Science, Technology and Innovation (CNPq/MCTI) through the Special Visiting Researcher fellowship [313772/2013-4]
The production of wax esters using microbial oils was demonstrated in this study. Microbial oils produced from food waste and by-product streams by three oleaginous yeasts were converted into wax esters via enzymatic catalysis. Palm oil was initially used to evaluate the influence of temperature and enzyme activity on wax ester synthesis catalysed by Novozyme 435 and Lipozyme lipases using cetyl, oleyl and behenyl alcohols. The highest conversion yields (up to 79.6%) were achieved using 4 U/g of Novozyme 435 at 70 degrees C. Transesterification of microbial oils to behenyl and cetyl esters was achieved at conversion yields up to 87.3% and 69.1%, respectively. Novozyme 435 was efficiently reused for six and three cycles during palm esters and microbial esters synthesis, respectively. The physicochemical properties of microbial oil derived behenyl esters were comparable to natural waxes. Wax esters from microbial oils have potential applications in cosmetics, chemical and food industries.
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