期刊
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
卷 21, 期 12, 页码 721-732出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/omi.2017.0143
关键词
metabolomics; Wolfram syndrome; wolframin; diabetes; endoplasmic reticulum stress
资金
- Institutional Research Funding [IUT20-42, PUT1416]
- Center of Excellence for Genomics and Translational Medicine from the Estonian Ministry of Education and Science
- European Union through the European Regional Development Fund [2014-2020.4.01.15-0012]
Wolfram syndrome 1 is a rare autosomal recessive neurodegenerative disease characterized by diabetes insipidus, diabetes mellitus, optic atrophy, and deafness. Mutations in the WFS1 gene encoding the wolframin glycoprotein can lead to endoplasmic reticulum stress and unfolded protein responses in cells, but the pathophysiology at whole organism level is poorly understood. In this study, several organs (heart, liver, kidneys, and pancreas) and bodily fluids (trunk blood and urine) of 2- and 6-month old Wfs1 knockout (KO), heterozygote (HZ), and wild-type (WT) mice were analyzed by untargeted and targeted metabolomics using liquid chromatography-mass spectrometry. The key findings were significant perturbations in the metabolism of pancreas and heart before the onset of related clinical signs such as glycosuria that precedes hyperglycemia and thus implies a kidney dysfunction before the onset of classical diabetic nephropathy. The glucose use and gluconeogenesis in KO mice are intensified in early stages, but later the energetic needs are mainly covered by lipolysis. Furthermore, in young mice liver and trunk blood hypouricemia, which in time turns to hyperuricemia, was detected. In summary, we show that the metabolism in Wfs1-deficient mice markedly differs from the metabolism of WT mice in many aspects and discuss the future biological and clinical relevance of these observations.
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