期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
卷 24, 期 -, 页码 139-145出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbd.2016.03.001
关键词
Adelphocoris suturalis Jakovlev; Chemosensory protein; Next generation sequencing; Odorant-binding protein; Quantitative real-time PCR
资金
- China National 973 Basic Research Program [2012CB114104]
- National Natural Science Foundation of China [31272048, 31321004, 31401737, 31471778]
- Beijing talents fund [2015000021223ZK29]
- Beijing Nova Program [Z1511000003150118]
Adelphocoris suturalis Jakovlev (Hemiptera: Miridae) is an insect pest that causes severe agricultural damage to cotton and many other important crops. In insects, olfaction is very important throughout their lifetime. There are two groups of small soluble proteins, named odorant binding proteins (OBPs) and chemosensory proteins (CSPs), which are suggested to participate in the initial biochemical recognition steps of insect olfactory signal transduction. In this study, a total of 16 OBPs (12 classical OBPs and 4 plus-C OBPs) and 8 CSPs, were identified in the antennal transcriptome of A. suturalis. The sex- and tissue-specific profiles of these binding protein genes showed that 13 of the 16 OBP transcripts were highly expressed in the antennae of both sexes, and 4 OBPs (AsutOBP1, 4, 5 and 9) were expressed higher in the male antennae compared to the female antennae. Three CSPs (AsutCSP1, 4 and 5) were expressed specifically in the antennae of both sexes, and AsutCSP1 was expressed higher in the male antennae than in the female antennae. Our findings identify several novel OBP and CSP genes for further investigation of the olfactory system of A. suturalis at the molecular level. (C) 2016 Published by Elsevier Inc.
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