期刊
CIRCULATION
卷 136, 期 23, 页码 2248-+出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCULATIONAHA.117.030235
关键词
cardiomyopathy; FUNDC1; heart failure; IP(3)R2; MAMs
资金
- National Heart Lung and Blood Institute [HL079584, HL080499, HL089920, HL110488, HL128014, HL132500, HL137371]
- National Cancer Institute [CA213022]
- National Institute on Aging [AG047776]
- American Heart Association (AHA) [16GRANT29590003]
- American Heart Association postdoctoral fellowship award [16POST29960011, 16POST31160009]
- Ministry of Science and Technology of China [2016 YFA0101100]
BACKGROUND: FUN14 domain containing 1 (FUNDC1) is a highly conserved outer mitochondrial membrane protein. The aim of this study is to examine whether FUNDC1 modulates the mitochondrial-associated endoplasmic reticulum (ER) membranes (MAMs), mitochondrial morphology, and function in cardiomyocytes and intact hearts. METHODS: The impacts of FUNDC1 on MAMs formation and cardiac functions were studied in mouse neonatal cardiomyocytes, in mice with cardiomyocyte-specific Fundc1 gene knockout (Fundc1(f/Y)/Cre alpha(MyHC+/-)), and in the cardiac tissues of the patients with heart failure. RESULTS: In mouse neonatal cardiomyocytes and intact hearts, FUNDC1 was localized in MAMs by binding to ER-resided inositol 1,4,5-trisphosphate type 2 receptor (IP(3)R2). Fundc1 ablation disrupted MAMs and reduced the levels of IP(3)R2 and Ca2+ in both mitochondria and cytosol, whereas overexpression of Fundc1 increased the levels of IP(3)R2 and Ca2+ in both mitochondria and cytosol. Consistently, Fundc1 ablation increased Ca2+ levels in ER, whereas Fundc1 overexpression lowered ER Ca2+ levels. Further, Fundc1 ablation in cardiomyocytes elongated mitochondria and compromised mitochondrial functions. Mechanistically, we found that Fundc1 ablation-induced reduction of intracellular Ca2+ levels suppressed mitochondrial fission 1 protein (Fis1) expression and mitochondrial fission by reducing the binding of the cAMP response element binding protein (CREB) in the Fis1 promoter. Fundc1(f/Y)/Cre(alpha MyHC+/)-mice but not their littermate control mice (Fundc1(f/Y)/Cre(alpha MyHC+/-)) exhibited cardiac dysfunction. The ligation of the left ventricle artery of Fundc1(f/Y)/Cre(alpha MyHC+/-)mice caused more severe cardiac dysfunction than those in sham-treated Fundc1(f/Y)/Cre(alpha MyHC+/-)mice. Finally, we found that the FUNDC1/MAMs/CREB/Fis1 signaling axis was significantly suppressed in patients with heart failure. CONCLUSIONS: We conclude that FUNDC1 binds to IP(3)R2 to modulate ER Ca2+ release into mitochondria and cytosol. Further, a disruption of the FUNDC1 and IP(3)R2 interaction lowers the levels of Ca2+ in mitochondria and cytosol, both of which instigate aberrant mitochondrial fission, mitochondrial dysfunction, cardiac dysfunction, and heart failure.
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