期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 48, 页码 41760-41766出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b15516
关键词
nanocontainer; self-assembly; molecular recognition; surface design; stimulus-responsive; immobilization
资金
- Fonds der Chemischen Industrie
- Deutsche Forschungsgemeinschaft [DFG SFB858, EXC 1003]
We present the preparation of ligand-conjugated redox-responsive polymer nanocontainers by the supramolecular decoration of cydodextrin vesicles with a thin redox-cleavable polymer shell that displays molecular recognition units on its surface. Two widely different recognition motifs (mannose-Concanavalin A and biotin-streptavidin) are compared and the impact of ligand density on the nanocontainer surface as well as an additional functionalization with nonadhesive poly(ethylene glycol) is studied. Aggregation assays, dynamic light scattering, and a fluorometric quantification reveal that the molecular recognition of ligand-conjugated polymer nanocontainers by receptor proteins is strongly affected by the multivalency of interactions and the association strength of the recognition motif. Finally, microcontact printing is used to prepare streptavidin-patterned surfaces, and the specific immobilization of biotin-conjugated nanocontainers is demonstrated. As a prototype of a nanosensor, these tethered nanocontainers can sense a reductive environment and react by releasing a payload.
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