期刊
BIOCONJUGATE CHEMISTRY
卷 28, 期 11, 页码 2729-2736出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.7b00483
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资金
- National Science Foundation (NSF) [CBET-0931875, CBET-1337061]
- URI Genomics Sequencing Center (RI NSF EPSCoR) [OIA-1004057]
- MRI Facility in the Brain Institute at Brown University
- Electron Microscopy Facilities in the RI Consortium for Nanoscience and Nanotechnology
- National Institutes of Health
- National Heart, Lung and Blood Institute [R01HL101977]
- Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) from National Institute of General Medical Sciences) [P20GM103430]
We present an approach to tuning the multifunctionality of iron oxide nanoparticles (IONs) using mixed self-assembled monolayers of cationic lipid and anionic polyethylene glycol (PEG) lipid. By forming stable, monodispersed lipid-coated IONs (L-IONs) through a solvent-exchange technique, we were able to demonstrate the relationship between surface charge, the magnetic transverse relaxivity (r(2) from T-2-weighted images), and the binding capacity of small interfering ribonucleic acids (siRNAs) as a function of the cationic-to-anionic (PEG) lipid ratio. These properties were controlled by the cationic charge and the PEG conformation; relaxivity and siRNA binding could be varied in the mushroom and brush regimes but not at high brush densities. In vitro results combining cell viability, uptake, and transfection efficiency using HeLa cells suggest that the functional physicochemical and biological properties of L-IONs may be best achieved using catanionic lipid coatings near equimolar ratios of cationic to anionic PEG-lipids.
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