期刊
ANIMAL BIOTECHNOLOGY
卷 28, 期 4, 页码 237-241出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2016.1267016
关键词
Cytokines; Inflammatory responses; LPS; Porcine; TREM-1
资金
- Natural Science Foundation of Jiangsu Province [BK20151365]
- National Science Foundation of China [31402080]
- Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province [CX (13)5039]
Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-) caused by LPS stimulation in the cultured porcine alveolar macrophages. These results indicate that the recombinant porcine sTREM-1 protein can prevent TREM-1-mediated hyperinflammatory responses after exposure to LPS.
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