期刊
TRANSGENIC RESEARCH
卷 26, 期 3, 页码 375-384出版社
SPRINGER
DOI: 10.1007/s11248-017-0013-6
关键词
Plant suspension cells; Antibody; N-Glycosylation; beta-1,4-Galactosyltransferase
类别
资金
- Service Public de Wallonie (WBHealth) [1318062]
- Belgian Fund for Scientific Research
- European Fund for Economic and Regional Development (GIGA-Proteomics facility instrumentation)
- Walloon Region
Nicotiana tabacum BY-2 suspension cells have several advantages that make them suitable for the production of full-size monoclonal antibodies which can be purified directly from the culture medium. Carbohydrate characterization of an antibody (Lo-BM2) expressed in N. tabacum BY-2 cells showed that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (> 70%) of the complex GnGnXF glycoform. The stable co-expression of a human beta-1,4-galactosyltransferase targeted to different Golgi sub-compartments altered Lo-BM2N-glycosylation and resulted in the production of an antibody that exhibited either hybrid structures containing a low abundance of the plant epitopes (alpha-1,3-fucose and beta-1,2-xylose), or a large amount of galactose-extended N-glycan structures. These results demonstrate the suitability of stable N-glycoengineered N. tabacum BY-2 cell lines for the production of human-like antibodies.
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