COMBINING GLOBAL TRACKING WITH STATISTICAL CLASSIFICATION TO ANALYZE ENDOCYTOSIS DYNAMICS USING TIRF MICROSCOPY

In this work we set several mathematical tools to study the role of Dynamin and Endophilin in the Clathrin-mediated endocytosis process. Their different dynamics and co-localizations are observed by using TIRF microscopy. We define in particular a novel tracking method in order to track the Clathrin-coated pits and quantify their co-localization with the proteins involved in the process. Thereafter, we perform a statistical analysis of the lifetimes of Clathrin-coated pits tracks by a suitable mixture model. This allows to define different populations of interest and point out the role of Endophilin and Dynamin in the process.