期刊
EUROPEAN JOURNAL OF MEDICAL RESEARCH
卷 22, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s40001-017-0294-5
关键词
Colon cancer; MicroRNA-gene microarray; Molecular marker expression profile; Genetic diagnosis
资金
- Suzhou Science and Education Project-Youth Science and Technology Foundation [KJXW2014017]
Purpose: To identify the expression profile of novel microRNAs (miRNAs) in colon cancer and evaluate their clinical applicability. Methods: Differences in the expression of serum miRNAs in patients with colon cancer and healthy controls were identified using miRNA microarrays. Differentially expressed miRNAs were verified via real-time polymerase chain reaction (PCR) using sera from 50 patients with colon cancer and 44 healthy controls. These miRNAs were also verified in a double-blind validation experiment using sera from 30 patients with colon cancer, 30 patients with colonic polyps, and 30 healthy controls. Results: Microarray hybridization revealed that 87 miRNAs were differentially expressed between the sera of patients with colon cancer and healthy controls. Among these miRNAs, 39 miRNAs were up-regulated, whereas 48 miRNAs were down-regulated. Verification of the expression of these miRNAs using real-time PCR revealed that the expression levels of miR-31, miR-141, miR-224-3p, miR-576-5p, and miR-4669 were significantly different between patients with colon cancer and healthy controls. Using these five miRNAs to construct a miRNA expression profile (or miRNA panel) will facilitate more effective diagnosis of colon cancer. Conclusion: Clinical analysis of miR-31, miR-141, miR-224-3p, miR-576-5p, and miR-4669 expression in patients with colon cancer may facilitate the diagnosis of colon cancer.
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