Characterization of a novel mosquitocidal toxin of Cry50Ba and its potential synergism with other mosquitocidal toxins

A putative toxin gene of cry50Ba was successfully expressed in E. coli cells and confirmed that the purified Cry50Ba toxin had very high toxic activity against Culex quinquefasciatus larvae. Furthermore, the potential synergism of Cry50Ba toxin with Cry2Aa, Cry4Aa and CryllAa at a ratio of 1:1 was investigated. Although no significant synergism with other toxins was observed, the Cry50Ba as a novel toxin could be used to delay rapid onset of resistance in mosquito. (C) 2017 Elsevier Ltd. All rights reserved.