期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 12, 期 24, 页码 3162-3171出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201701285
关键词
amino acids; immobilization; mesoporous materials; organosilicas; tyrosine-bridged organosilicas
资金
- National Natural Science Foundation of China [21106069]
- State Key Laboratory of Materials-Oriented Chemical Engineering [KL15-08]
New amino-acid-bridged periodic mesoporous organosilicas (PMOs) were constructed by hydrolysis and condensation reactions under acid conditions in the presence of a template. The tyrosine bissilylated organic precursor (TBOS) was first prepared through a multistep reaction by using tyrosine (a natural amino acid) as the starting material. PMOs with the tyrosine framework (Tyr-PMOs) were constructed by simultaneously using TBOS and tetraethoxysilane as complex silicon sources in the condensation process. All the Tyr-PMOs materials were characterized by XRD, FTIR spectroscopy, N-2 adsorption-desorption, TEM, SEM, and solid-state (SiNMR)-Si-29 spectroscopy to confirm the structure. The horseradish peroxidase (HRP) enzyme was first immobilized on these new Tyr-PMOs materials. Optimal conditions for enzyme adsorption included a temperature of 40 degrees C, a time of 8h, and a pH value of 7. Furthermore, the novel Tyr-PMOs materials could store HRP for approximately 40days and maintained the enzymatic activity, and the Tyr-PMOs-10%HRP with the best immobilization effect could be reused at least eight times.
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