期刊
CELL
卷 171, 期 7, 页码 1611-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2017.10.044
关键词
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资金
- American Academy of Otolaryngology Resident Grant
- New England Otolaryngology Society Resident Grant
- Human Frontiers Science Program
- NRSA
- Massachusetts Eye and Ear Infirmary
- National Cancer Institute
- NIH Common Fund
- Broad Institute
- Klarman Cell Observatory
- Starr Foundation
- Ludwig Center
- NIH
The diverse malignant, stromal, and immune cells in tumors affect growth, metastasis, and response to therapy. We profiled transcriptomes of similar to 6,000 single cells from 18 head and neck squamous cell carcinoma (HNSCC) patients, including five matched pairs of primary tumors and lymph node metastases. Stromal and immune cells had consistent expression programs across patients. Conversely, malignant cells varied within and between tumors in their expression of signatures related to cell cycle, stress, hypoxia, epithelial differentiation, and partial epithelial-to-mesenchymal transition (p-EMT). Cells expressing the p-EMT program spatially localized to the leading edge of primary tumors. By integrating single-cell transcriptomes with bulk expression profiles for hundreds of tumors, we refined HNSCC sub-types by their malignant and stromal composition and established p-EMT as an independent predictor of nodal metastasis, grade, and adverse pathologic features. Our results provide insight into the HNSCC ecosystem and define stromal interactions and a p-EMT program associated with metastasis.
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