期刊
CARBOHYDRATE POLYMERS
卷 178, 期 -, 页码 95-104出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.08.117
关键词
Pleurotus eryngii (King Oyster); Mannogalactan; Chemical structure; Antitumor; Melanoma B16-F10; Non-cytotoxic
资金
- CAPES (PROAP)
- CAPES (PROCAD)
- CNPq
A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii (King Oyster) basidiocarps and its biological properties were evaluated. Structural assignments were carried out using mono-and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. A man-nogalactan having a main chain of (1 -> 6)-linked alpha-D-galactopyranosyl and 3-O-methyl-alpha-D-galactopyranosyl residues, both partially substituted at OH-2 by beta-D-Manp (MG-Pe) single-unit was found. Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells. MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion. Also, 50 mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10 days decreased in 60% the tumor volume compared to control. Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed. Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
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