期刊
TOXICOLOGY IN VITRO
卷 40, 期 -, 页码 214-222出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2017.01.017
关键词
Nanosystem; Nanocapsules; Chitosan; Sperm; Spermtoxicology
类别
资金
- CNPq [310461/2012-0]
- CAPES
- FAPERGS
Toxicology studies have a pivotal role for selection of new nanosystems. As lipid -core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated With chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0 h and 1 h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCS and LNC-CS below 40% (v/v) concentration. (C) 2017 Elsevier Ltd. All rights reserved.
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