期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 50, 页码 15941-15944出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201706703
关键词
biomaterials; cryopreservation; ice recrystallization inhibitors; monolayers; polymers
资金
- ERC [CRYOMAT 638661, 278004 FreeCo]
- BBSRC [BB/F011199/1]
- Royal Society
- University of Warwick WCPRS
- EPSRC [MOAC DTC EP/F500378/1]
- Engineering and Physical Sciences Research Council [1359594] Funding Source: researchfish
- BBSRC [BB/F011199/1] Funding Source: UKRI
Tissue engineering, gene therapy, drug screening, and emerging regenerative medicine therapies are fundamentally reliant on high-quality adherent cell culture, but current methods to cryopreserve cells in this format can give low cell yields and require large volumes of solvent antifreezes. Herein, we report polyproline as a minimum (bio)synthetic mimic of antifreeze proteins that is accessible by solution, solid-phase, and recombinant methods. We demonstrate that polyproline has ice recrystallisation inhibition activity linked to its amphipathic helix and that it enhances the DMSO cryopreservation of adherent cell lines. Polyproline may be a versatile additive in the emerging field of macromolecular cryoprotectants.
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