期刊
THERIOGENOLOGY
卷 90, 期 -, 页码 147-152出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2016.11.027
关键词
Swine; Sperm; Intrauterine; Nulliparous; Insemination catheter
资金
- Agropecuaria El Escobar
- Agropecuaria La Fontana
- AGG Ganados
- Salvador Sevilla S.L.
- Ganados Pigapesa
- Spanish Ministry of Economy and Competitiveness (MINECO)
- European Regional Development Fund (FEDER) [AGL2015-66341-R, AGL2015-70159-P]
- Seneca Foundation of Murcia [20040/GERM/16]
Post-cervical (pC) artificial insemination (AI) has been successfully developed for application in multiparous sows, although it has proved problematic in gilts. This study analyzes the use of pC-Al in gilts by two experiments. In the first experiment, the efficiency of pC-Al in gilts was evaluated using a multi-ring multiparous catheter (MpC), which led to 23.1% of the gilts being successfully inseminated. In gilts where insemination was not possible using an MpC, two alternatives were applied before a second attempt at insemination: 1) Vetrabutin Chlorhydrate (VC) was intramuscularly injected in order to relax the cervix; or 2) Warm extender (WE) was deposited in the cervix to modify the cervical muscle dynamics. After the application of these treatments, the success rates achieved with the MpC were 34.2% and 23.8% for VC and WE, respectively. No statistically significant differences were found in the reproductive parameters measured [farrowing (%), litter size and fecundity index] between the use of MpC, or the MpC combined with VC or WE, compared with gilts inseminated by cervical Al (control group). In the second experiment, new catheters based on the anatomical characteristics of gilts (GpC) were used, and the rate of successful pC-Al application were compared (experiment 2a): a) MpC: control; b) GpC1: multi-ring catheter of empty set 16 mm and inner cannula of empty set3.5 mm; c) GpC2: a multi-ring catheter of 0 with an inner cannula of empty set2.5 mm. The highest rate of successful cannula penetration was reached in the GpC2 group (60.3%) followed by GpC1 (37.0%) and MpC (19.6%) (p < 0.05). There were no differences in the above mentioned reproductive parameters using the three catheters compared with cervical AI method (control group). Moreover, prior cervical AI did not improve subsequent pC-Al application 24 h later (experiment 2b). In conclusion, Vetrabutin Chlorhydrate, warm extender or the new catheters can be considered as useful tools for improving the success rate of pC-Al technique in gilts. (C)2016 Elsevier Inc. All rights reserved.
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