期刊
THERIOGENOLOGY
卷 100, 期 -, 页码 24-31出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2017.05.022
关键词
BAMBI; TGF-beta; Steroidogenesis; Granulosa cell
资金
- National Science and Technology Major Project of China [2016ZX08006003, 2014ZX08009-047B]
Transforming growth factor beta (TGF-beta), acting as the auto/para endocrine factors, has multi-function in mammalian follicle development. Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) is considered as a pseudoreceptor in the TGF-beta signal pathway which has the similar extra cellular structure of TGF-beta receptor but lack of intracellular serine/threonine kinase domains. However, the biological function of BAMBI involved in porcine granulosa cell steroidogenesis remains unknown. This study was thus carried out to explore the effect of BAMBI on the steroidogenesis process in porcine primary granulosa cells. Our results showed overexpression of BAMBI promoted aromatase and StAR, but not P450scc and 3 beta-HSD mRNA and protein expression levels in porcine primary granulosa cells, and increased the accumulation of estradiol and progesterone in the culture medium. Meanwhile, knockdown endogenous BAMBI decreased the mRNA expression levels of Cyp19a1 and Star and the accumulation levels of estradiol and progesterone. TGF-beta could decrease Cyp19a1 and Star mRNA expression and estradiol and progesterone production in a dose-dependent manner. Pre-treatment with BAMBI adenovirus reversed TGF beta 1-induced downregulation of Cyp19a1 and Star mRNA expression. Moreover, TGF-beta 1 could induce the phosphorylation of SMAD3 in porcine granulosa cells. Pre-transfected with BAMBI adenovirus also inhibited TGF-beta 1-induced downregulation of estradiol and progesterone production as well as TGF-beta 1-induced phosphorylation of SMAD3 in porcine granulosa cells. These findings provided a potential mechanism by which BAMBI could regulate porcine granulosa cell steroidogenesis. (C) 2017 Elsevier Inc. All rights reserved.
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