New Biosilified Pd -lipase hybrid biocatalysts for dynamic resolution of amines

In this work lipase CaLB was immobilized on functionalized Pd-SiO2 nanoparticles in order to simplify the DKR of alpha-methylbenzylamine. Hybrid biocatalysts showed immobilization efficiencies of 82%, 80% and 76% when containing 1, 5 and 10% of Pd respectively. On DKR reaction values of ee > 99% and conversion of 82% were found with only 1% of Pd, generating a productivity of 2.21 mg of product h(-1) mg of supporr1 against 0.76 found by N435((R)). Compared to commercial N435((R)), the novel biocatalysts showed protein loads about 15-fold lower and higher activity, demonstrating competitive performances and high industrial applications. (C) 2017 Elsevier Ltd. All rights reserved.